Mitsubishi P91W Instruction Manual Page 125
- Page / 144
- Table of contents
- TROUBLESHOOTING
- BOOKMARKS
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Installing Optional Accessories
115
2. Place the gel alignment template that matches the size of your sample
tray or agarose gel into the magnetic locator frame.
3. Place your gel or gel tray into the open area of the template.
Note: No recalibration is necessary to use the gel alignment
template kit.
Orange Fluorescence Reference Plate
The orange fluorescence reference plate allows you to apply UV flat fielding
corrections to your ChemiDoc MP imager. Corrections are made for all UV
illumination sources, filters, and the camera lens.
The orange fluorescence reference plate can be used to correct for image
nonuniformities for red and orange gels, including:
• Ethidium bromide
•GelRed
•Flamingo
®
fluorescent gel stain
• Coomassie Fluor Orange
• SYPRO Ruby protein blot stain
•Krypton
•Qdot 625
The orange fluorescence reference plate (catalog #170-8008) can be used
with several of the Bio-Rad Molecular Imager® series of products, including:
• ChemiDoc MP system with Image Lab software
• ChemiDoc XRS+ system with Image Lab software
• ChemiDoc XRS+ system with Quantity One™ software
When you order the reference plate, you receive:
• Orange fluorescence reference plate, overall dimensions: 29.5 x
29.5 cm; viewing surface: 27 x 27 cm
• Instruction sheet (10017296)
- ChemiDoc 1
- MP System 1
- Software 1
- Safety and Regulatory 3
- Compliance 3
- General Precautions 4
- Regulatory Notices 4
- Power Safety Information 4
- Contents 7
- 1 Introduction 11
- System Components 12
- Image Lab Software 13
- Emission Filters 13
- Optional Accessories 14
- System Requirements 15
- Computer Specifications 15
- NUCLEIC ACID GELS 16
- PROTEIN GELS 16
- Applications 17
- Hardware Specifications 17
- Operating Ranges 17
- Automation Capabilities 17
- ChemiDoc MP Imager Workflow 18
- For More Information 18
- 2 System Calibration 19
- 3 Image Lab 21
- Interface Overview 22
- Results Data 23
- Display Toolbox 24
- Start Page 24
- Analysis Tool Box 25
- Status Bar 26
- Menu Commands 26
- Interface Overview 27
- EDIT MENU COMMANDS 28
- 4 Acquiring Images 31
- The Protocol Setup Window 32
- Acquisition Settings 34
- Analyze Image 39
- STANDARDS 40
- STANDARD LANES 41
- REGRESSION METHODS 42
- Output Settings 43
- Review Protocol Settings 44
- APPLICATION OPTIONS 46
- Choosing a Protocol 50
- Editing Protocols 51
- Position Gel 52
- Running a Protocol 52
- Application Tables 55
- 5 Viewing Images 59
- Displaying Gel Images 60
- Zoom Tools 61
- Fit in Window 61
- Image Transform 62
- FOR MULTICHANNEL IMAGES 63
- Image Colors 64
- 3-D Projection 65
- Image Info 65
- IMAGE DETAILS 66
- ANALYSIS SETTINGS 66
- Multichannel View 67
- Splitting Multichannel Images 68
- CHANGE LAYOUT 69
- Displaying Data 70
- Analysis Table Options 71
- Lane and Band Definitions 72
- Volume Definitions 73
- Lane Profile 74
- Standard Curve 75
- 6 Analyzing Images 77
- DETECTION SETTINGS 78
- Analysis Toolbox Tools 79
- Image Tools 80
- INVERT DATA 81
- Lane and Band Tools 82
- ALL LANES 83
- SINGLE LANE 83
- LANE BACKGROUND SUBTRACTION 83
- BANDS TAB 84
- MOLECULAR WEIGHT STANDARD 86
- Quantity Tools 87
- ABSOLUTE QUANTITY TAB 89
- Annotation Tools 92
- TEXT PROPERTIES 93
- Volume Tools 95
- VOLUME TYPES 97
- VOLUME BACKGROUND SUBTRACTION 97
- RELATIVE VOLUME QUANTITY 98
- ABSOLUTE VOLUME QUANTITY 99
- ALIGNMENT 100
- 7 Generating Reports 101
- General Tab 102
- Lane and Band Table Tab 103
- Print Report to .pdf File 104
- Adjust the Printer Settings 104
- Vo l u me Ta b le Tab 104
- Print Report 104
- 8 Exporting Results 105
- Exporting Gel Images 107
- Analysis Table Export 109
- 9 Maintenance 111
- Fuse Replacement 113
- Appendix A. Using the 115
- Criterion Stain Free 115
- Stain-Free Workflow 116
- Imaging Gels 117
- Imaging Blots 117
- Appendix B. Troubleshooting 119
- Appendix C. Accessories 121
- XcitaBlue™ Conversion Screen 122
- Gel Alignment Template Kit 124
- FLUORESCENCE REFERENCE PLATE 126
- Ordering Information 127
- Appendix D. Mitsubishi P93/ 131
- P95 Thermal Printer 131
- Settings 131
- Appendix E. Regression 135
- Calculation Methods 135
- Glossary 137
- Zoom tools 143
- Life Science 144
- Bio-Rad 144
- Laboratories, Inc 144
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