Mitsubishi P91W Instruction Manual download pdf (Page 37)

Creating a Single-Channel Protocol

prominent bands. Exposure time can range from 0.001 to 7200

seconds with the ChemiDoc™ MP system

After imaging a gel with automatic exposure optimization, the

exposure time displays in the protocol (see below) so you can

manually adjust the exposure time if needed.

Note: You can also view the exposure time of the image later, in

the Image Info window (see Image Info on page 55).

Signal Accumulation Mode—if you are running a chemiluminescent

application on the ChemiDoc MP system, you can also use signal

accumulation mode (SAM).

SAM is used to simplify capture of a good image from a

chemiluminescent sample. This sample type often requires long

integration times to obtain an image that represents the best range of

signal.

Rather than manually acquiring a series of independent images with

different imaging times, SAM presents a series of cumulative images

with progressively greater signal in each image. SAM requires you to

estimate the shortest and longest times expected to generate an image

with the appropriate signal intensity. You then decide how many total

images to acquire in this window of time.

For example, if the minimum time that is expected to image the sample

is 1 min and the maximum is 5 min, these values are entered (in

seconds) in the setup window. By entering 5 in the Total number of

images field, three images will be acquired between the first and last

images.

To use SAM, click Signal Accumulation Mode and select Setup to

display the Signal Accumulation Setup dialog box.

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Mitsubishi P91W Instruction Manual Page 37