Mitsubishi P91W Instruction Manual Page 8
- Page / 144
- Table of contents
- TROUBLESHOOTING
- BOOKMARKS
Rated. / 5. Based on customer reviews
ChemiDoc MP with Image Lab Software| Contents
viii
Output Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Review Protocol Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Creating a Multichannel Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Acquisition Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Output Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Choosing a Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Editing Protocols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Position Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Running a Protocol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Setting Up a Custom Application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Application Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Creating a Multichannel Image from Single Images . . . . . . . . . . . . . . . . . . . . . 47
Chapter 5 Viewing Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Displaying Gel Images. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Display Gel Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
Zoom Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Fit in Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Image Colors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
3-D Projection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Image Info . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Displaying Multichannel Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Multichannel View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Splitting Multichannel Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
Displaying Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Analysis Table Options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Lane and Band Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Volume Definitions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Lane Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Standard Curve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Chapter 6 Analyzing Images . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Auto Analysis Settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Analysis Toolbox Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Image Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Lane and Band Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Molecular Weight Analysis Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Quantity Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Annotation Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
- ChemiDoc 1
- MP System 1
- Software 1
- Safety and Regulatory 3
- Compliance 3
- General Precautions 4
- Regulatory Notices 4
- Power Safety Information 4
- Contents 7
- 1 Introduction 11
- System Components 12
- Image Lab Software 13
- Emission Filters 13
- Optional Accessories 14
- System Requirements 15
- Computer Specifications 15
- NUCLEIC ACID GELS 16
- PROTEIN GELS 16
- Applications 17
- Hardware Specifications 17
- Operating Ranges 17
- Automation Capabilities 17
- ChemiDoc MP Imager Workflow 18
- For More Information 18
- 2 System Calibration 19
- 3 Image Lab 21
- Interface Overview 22
- Results Data 23
- Display Toolbox 24
- Start Page 24
- Analysis Tool Box 25
- Status Bar 26
- Menu Commands 26
- Interface Overview 27
- EDIT MENU COMMANDS 28
- 4 Acquiring Images 31
- The Protocol Setup Window 32
- Acquisition Settings 34
- Analyze Image 39
- STANDARDS 40
- STANDARD LANES 41
- REGRESSION METHODS 42
- Output Settings 43
- Review Protocol Settings 44
- APPLICATION OPTIONS 46
- Choosing a Protocol 50
- Editing Protocols 51
- Position Gel 52
- Running a Protocol 52
- Application Tables 55
- 5 Viewing Images 59
- Displaying Gel Images 60
- Zoom Tools 61
- Fit in Window 61
- Image Transform 62
- FOR MULTICHANNEL IMAGES 63
- Image Colors 64
- 3-D Projection 65
- Image Info 65
- IMAGE DETAILS 66
- ANALYSIS SETTINGS 66
- Multichannel View 67
- Splitting Multichannel Images 68
- CHANGE LAYOUT 69
- Displaying Data 70
- Analysis Table Options 71
- Lane and Band Definitions 72
- Volume Definitions 73
- Lane Profile 74
- Standard Curve 75
- 6 Analyzing Images 77
- DETECTION SETTINGS 78
- Analysis Toolbox Tools 79
- Image Tools 80
- INVERT DATA 81
- Lane and Band Tools 82
- ALL LANES 83
- SINGLE LANE 83
- LANE BACKGROUND SUBTRACTION 83
- BANDS TAB 84
- MOLECULAR WEIGHT STANDARD 86
- Quantity Tools 87
- ABSOLUTE QUANTITY TAB 89
- Annotation Tools 92
- TEXT PROPERTIES 93
- Volume Tools 95
- VOLUME TYPES 97
- VOLUME BACKGROUND SUBTRACTION 97
- RELATIVE VOLUME QUANTITY 98
- ABSOLUTE VOLUME QUANTITY 99
- ALIGNMENT 100
- 7 Generating Reports 101
- General Tab 102
- Lane and Band Table Tab 103
- Print Report to .pdf File 104
- Adjust the Printer Settings 104
- Vo l u me Ta b le Tab 104
- Print Report 104
- 8 Exporting Results 105
- Exporting Gel Images 107
- Analysis Table Export 109
- 9 Maintenance 111
- Fuse Replacement 113
- Appendix A. Using the 115
- Criterion Stain Free 115
- Stain-Free Workflow 116
- Imaging Gels 117
- Imaging Blots 117
- Appendix B. Troubleshooting 119
- Appendix C. Accessories 121
- XcitaBlue™ Conversion Screen 122
- Gel Alignment Template Kit 124
- FLUORESCENCE REFERENCE PLATE 126
- Ordering Information 127
- Appendix D. Mitsubishi P93/ 131
- P95 Thermal Printer 131
- Settings 131
- Appendix E. Regression 135
- Calculation Methods 135
- Glossary 137
- Zoom tools 143
- Life Science 144
- Bio-Rad 144
- Laboratories, Inc 144
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