Mitsubishi P91W Instruction Manual download pdf (Page 135)

Appendix E: Regression Calculation Methods

125

Appendix E. Regression

Calculation Methods

Each regression method calculates a standard curve. Some of the methods

provide the formula for the standard curve. In this case, the molecular weight

can be calculated by:

x = relative front of the band of interest

y = molecular weight of the band of interest

Linear (semilog): The linear equation is y = a + bx, where a is the intercept

and b is the slope of the line.

Note: The linear equation is calculated on the log of the

molecular weight values.

The R

value may be used to determine the overall quality of the linear fit. A

linear regression with an R

value of >0.99 is considered a very good fit. The

primary advantage of this method is that it is extremely simple. The primary

disadvantage is that it will deliver incorrect results if the data are not very

linear.

Point-to-point (semilog): No single equation is available for the point-to-

point method. The slope of each segment of the curve between data points is

calculated independently.

Note: The log of the molecular weight values is used to calculate

the slope for each segment of the curve.

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Mitsubishi P91W Instruction Manual Page 135