ChemiDoc MP System with Image Lab Software
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• Elimination of the need for acetic acid and methanol in staining and
destaining, which reduces organic waste
• Visualization of transferred or blotted proteins on low fluorescence
PVDF membranes
Stain-Free Workflow
For detailed information about workflow steps, refer to the Criterion™ Precast
Gels Instruction Manual and Application Guide or to the Mini-PROTEAN®
Precast Gels Instruction Manual and Application Guide, except where
otherwise noted.
Electrophoresis with Stain-Free Gels
Stain-free gels are made and packaged without SDS so they can be used for
both SDS and native PAGE applications.
To perform electrophoresis with these gels, prepare the sample and running
buffers, set up the electrophoresis cell, and perform the run.
See Chapter 4, Acquiring Images
Prepare buffers
Prepare gels and assemble
electrophoresis cell
Prepare and load samples
Perform electrophoresis
Activate/image gels
Analyze the separation
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