Mitsubishi P91W Instruction Manual download pdf (Page 86)

ChemiDoc MP with Image Lab Software | Analyzing Images

Molecular Weight Analysis Tools

Molecular Weight Analysis Tools enable you to determine molecular weight (or

base pairs, if using nucleic acid gels) by comparing a test sample with known

standards.

You can view each band’s molecular weight in the molecular weight column of

the Lane and Band tab in the Analysis Table view or in the gel image by

selecting Mol. Wt. from the Band Attributes section of the Display Gel Options

window:

MOLECULAR WEIGHT STANDARD

You can change the standards that determine the molecular weight of test

samples.

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ChemiDoc 1
MP System 1
Software 1
Safety and Regulatory 3
Compliance 3
General Precautions 4
Regulatory Notices 4
Power Safety Information 4
Contents 7
1 Introduction 11
System Components 12
Image Lab Software 13
Emission Filters 13
Optional Accessories 14
System Requirements 15
Computer Specifications 15
NUCLEIC ACID GELS 16
PROTEIN GELS 16
Applications 17
Hardware Specifications 17
Operating Ranges 17
Automation Capabilities 17
ChemiDoc MP Imager Workflow 18
For More Information 18
2 System Calibration 19
3 Image Lab 21
Interface Overview 22
Results Data 23
Display Toolbox 24
Start Page 24
Analysis Tool Box 25
Status Bar 26
Menu Commands 26
Interface Overview 27
EDIT MENU COMMANDS 28
4 Acquiring Images 31
The Protocol Setup Window 32
Acquisition Settings 34
Analyze Image 39
STANDARDS 40
STANDARD LANES 41
REGRESSION METHODS 42
Output Settings 43
Review Protocol Settings 44
APPLICATION OPTIONS 46
Choosing a Protocol 50
Editing Protocols 51
Position Gel 52
Running a Protocol 52
Application Tables 55
5 Viewing Images 59
Displaying Gel Images 60
Zoom Tools 61
Fit in Window 61
Image Transform 62
FOR MULTICHANNEL IMAGES 63
Image Colors 64
3-D Projection 65
Image Info 65
IMAGE DETAILS 66
ANALYSIS SETTINGS 66
Multichannel View 67
Splitting Multichannel Images 68
CHANGE LAYOUT 69
Displaying Data 70
Analysis Table Options 71
Lane and Band Definitions 72
Volume Definitions 73
Lane Profile 74
Standard Curve 75
6 Analyzing Images 77
DETECTION SETTINGS 78
Analysis Toolbox Tools 79
Image Tools 80
INVERT DATA 81
Lane and Band Tools 82
ALL LANES 83
SINGLE LANE 83
LANE BACKGROUND SUBTRACTION 83
BANDS TAB 84
MOLECULAR WEIGHT STANDARD 86
Quantity Tools 87
ABSOLUTE QUANTITY TAB 89
Annotation Tools 92
TEXT PROPERTIES 93
Volume Tools 95
VOLUME TYPES 97
VOLUME BACKGROUND SUBTRACTION 97
RELATIVE VOLUME QUANTITY 98
ABSOLUTE VOLUME QUANTITY 99
ALIGNMENT 100
7 Generating Reports 101
General Tab 102
Lane and Band Table Tab 103
Print Report to .pdf File 104
Adjust the Printer Settings 104
Vo l u me Ta b le Tab 104
Print Report 104
8 Exporting Results 105
Exporting Gel Images 107
Analysis Table Export 109
9 Maintenance 111
Fuse Replacement 113
Appendix A. Using the 115
Criterion Stain Free 115
Stain-Free Workflow 116
Imaging Gels 117
Imaging Blots 117
Appendix B. Troubleshooting 119
Appendix C. Accessories 121
XcitaBlue™ Conversion Screen 122
Gel Alignment Template Kit 124
FLUORESCENCE REFERENCE PLATE 126
Ordering Information 127
Appendix D. Mitsubishi P93/ 131
P95 Thermal Printer 131
Settings 131
Appendix E. Regression 135
Calculation Methods 135
Glossary 137
Zoom tools 143
Life Science 144
Bio-Rad 144
Laboratories, Inc 144

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