Mitsubishi P91W Instruction Manual download pdf (Page 39)

Creating a Single-Channel Protocol

Analyze Image

STEP 2. DETECT LANES AND BANDS

To analyze the gel or blot, Image Lab software must detect lanes and

bands on the image. Lanes are detected automatically, and then the

background is subtracted automatically. Refer to Lane and Band Tools

for details. Customize band detection with the following options.

9. Select the Lane and Band checkbox in the left pane of the Protocol

Setup window. The right pane displays lane and band detection

options.

• Low Band Detection Sensitivity—this option sets detection at a

low level for images with more prominent bands. Faint bands are

not detected with this setting

• High Band Detection Sensitivity—this option sets detection at a

higher level for images that are more faint. Extraneous bands can

be removed later, using the Band Tools in the Analysis Toolbox.

See Lane and Band Tools on page 72

• Custom—select a numeric value between 1 and 100 to choose the

best detection sensitivity for your sample

Note: When Low Band Detection Sensitivity or High Band

Detection Sensitivity is used, numerical values are set: low

sensitivity = 25; high sensitivity = 75.

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Mitsubishi P91W Instruction Manual Page 39