Mitsubishi P91DW User Manual download pdf (Page 105)

Alpha Innotech User’s Manual, version 4

5-4

A Skewed Template

Specifying the Areas to be Measured

For microtiter plate analysis, the toolbox controls labeled Center and Outer specify the

size of the inner circle and outer torus scanned to measure each well. The number in the

center of each of these controls indicates the diameter of the circles. Click on an arrow to

increase or decrease the diameters. As the number is adjusted, the objects on the

template grow larger or smaller, reflecting the changes.

When specifying the size of the area to be measured, exclude shadows or other imaging

artifacts. Depending upon the lighting conditions when the image was captured, there

may be a crescent-shaped shadow obscuring part of the bottom of the wells, especially

those near the edge of the microtiter plate. We recommend decreasing the size of the

sampling area so that these regions are not included.

To measure the total density within each object, reduce the Center value to zero (0) and

adjust the Outer value until the objects on the screen are slightly smaller than the areas of

interest on the image. Reposition the corner objects using the mouse until the objects are

properly aligned over the objects.

Measuring Density

Once the objects are sized and positioned correctly, click on the SCAN button in the tool

box. The density values will be calculated and displayed under each object.

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Mitsubishi P91DW User Manual Page 105