Mitsubishi P91DW User Manual download pdf (Page 116)

Chapter 5: Analysis Tools

5-15

REVERSE button described in Chapter 3, this function does not alter the appearance of

the image.

This function is especially important when using Snap to Peak. If INVERT is

incorrectly set, the cursor will snap to areas between peaks rather than finding peaks.

Calculating R

Values

To obtain accurate R

values, specify the location of the wells (i.e., origin) and the dye

front using the functions Set Well Pos./Start and Set Dye Front/End both found in the

Marker menu.

To designate the location of the wells, point the cursor at Set Well Pos./Start. Position

the cursor so it is pointing anywhere along the wells and click the left mouse button. A

horizontal bar appears, defining the location of the origin. By clicking and dragging, the

bar can be moved for better positioning.

Repeat this procedure using Set Dye Front/End to indicate the location of the dye front.

Once the origin and dye front have been defined, they are used to calculate the R

values

of any bands that are added. If dye fronts are not assigned, AlphaEaseFC uses the top

and bottom of the image as the boundaries for calculating R

values.

The Molecular Weight Cursor Box

Molecular Weight Cursor Box

The MW. CURSOR box, found in the Molecular Weight toolbox, reports the location

on the y-axis, the molecular weight and the R

value of the current position of the cursor.

These data are updated as the cursor is moved. This information can be helpful for

positioning the cursor in a precise location, or for a quick estimate of a band’s size.

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Mitsubishi P91DW User Manual Page 116