Mitsubishi P91DW User Manual download pdf (Page 181)

Alpha Innotech User’s Manual, version 4

5-80

open the circle. When the circle corresponds to the perimeter of the Petri dish, release the

mouse. The circle has “handles” around it, and can be moved or re-sized at this point.

Hint: to draw a perfect circle around a portion of an image, first

visualize a square surrounding the area of interest. Position the mouse

in the upper left hand corner of the square. Click and drag the mouse

down across the area of interest at a 45° angle until the circle encloses

the area of interest.

Rectangles: After clicking on the button in the toolbox labeled with a square, move to

one of the four corners of the region to be enclosed. Click the left mouse button and

move the cursor to the corner diagonally opposite. Note the box that opens as the cursor

moves. Release the left mouse button when the box has reached the desired size. The

rectangle has “handles” around it, and can be moved or re-sized at this point.

Freehand Drawings: To draw a freehand object, select the freehand icon; the mouse will

change to a pencil. Draw an object of the desired size and shape. When the mouse is

released, the start and end points of the object will be connected.

Manipulating Areas of Interest: Areas of interest can be moved, copied and deleted.

To perform any of these functions, an area must first be selected by clicking on it or

dragging the cursor around it. Its color changes to gray, indicating that it is active. More

than one area of interest can be selected at any given time.

To move an area of interest click on the selected area. While holding down the left

mouse button, drag the area to the desired location.

To delete an area of interest click on the cut button in the toolbox (designated by a pair of

scissors). The selected area of interest is removed and any other areas of interest are

renumbered accordingly.

To copy a selected area of interest, click on the copy button (found next to the cut

button). A second copy of the area of interest appears overlapping the first one. Click

and drag the copy to the desired location.

2. Set the Density Threshold(s)

The objects counted by the automatic counting routine are based on the difference in the

gray levels of the objects as compared to the background (e.g., the colonies on the petri

dish are either darker or lighter than the surrounding area). The density thresholds define

the gray levels that are recognized by the counting routine.

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Mitsubishi P91DW User Manual Page 181