Mitsubishi P91DW User Manual download pdf (Page 119)

Alpha Innotech User’s Manual, version 4

5-18

Setting up the Lane Template

When the 1D-Multi button is clicked, a lane template appears on the image. The red lines

indicate the borders of adjacent lanes. The green lines define the Scan Width (described

below).

Under the heading Grid Controls, the number of lanes can be specified. The number

under the title Lanes indicates the number of lanes and can be changed by clicking the

right (increase) or left (decrease) arrows. As the lane number is adjusted, the template

displayed over the image is updated to reflect the changes.

Once the number of lanes has been set, the template should be adjusted so it coincides

with the lanes on the image.

If the lanes on the gel are horizontally oriented (sideways) the template can be rotated.

To change the orientation of the template to coincide with the image, click the ROTATE

checkbox. The template will rotate 90° counterclockwise. Clicking in the check box

again ("X" disappears) rotates the template 90° clockwise, restoring it to its original

orientation.

After specifying the correct number of lanes and setting the orientation, use the mouse to

drag the outside borders of the template so they frame the lanes to be scanned. Clicking

on or within any border repositions the template. Clicking on the blue “handles”

surrounding the template resizes it.

If the lanes of the gel are not perfectly straight, click the SKEWING checkbox. The

template will change to show four “handles” -- one at each corner of the template.

Position each corner of the template until it is positioned appropriately.

Adjust the template until the border of the template frames the lanes to be scanned and

the red lines lie between each of the lanes.

Skewed 1D-Multi Template Properly Aligned on a Gel

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Mitsubishi P91DW User Manual Page 119