Mitsubishi P91DW User Manual download pdf (Page 79)

Alpha Innotech User’s Manual, version 4

4-4

A Coomassie blue-stained protein gel visualized with a white light box has a histogram

reflecting mostly bright pixels:

Histogram of a typical Coomassie gel

Most of the pixels are found in the light portion of this histogram. The dark bands

represent a small number of pixels and include a variety of gray values, and therefore do

not show up as a single peak.

The histogram function is particularly useful to verify that an image spans the maximum

range of gray levels. When an image is to be used for analysis, it is especially important

that the gray level range be as large as possible. If an image does not include most of the

gray levels, we recommend repeating the image capturing process.

4.4 Automatic Enhancement

The Enhance Tools

The Enhance tool is found in Tool Box, Enhancement Tools. This function is ideal

for new or inexperienced users of the system since it offers 9 levels of automatic image

enhancement of the black, white, and gamma levels simultaneously. For an

inexperienced user, in can be difficult to adjust each black, white, and gamma buttons to

their respective optimal positions. By clicking on one of the nine Auto Enhance Level

buttons, the image is optimized according to a unique level. Button 1 will make the

image ‘darker’. Each increasing button click will ‘lighten’ up the image until button 9 is

pressed which will make the image the ‘lightest’ possible.

To undo any Auto Enhance Levels, just press on the Reset button on the main

interface.

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Mitsubishi P91DW User Manual Page 79