Chapter 5: Analysis Tools
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To add a second object, click on the right mouse button to re-enter “add” mode, then
click on the desired object with the left mouse button.
Using the Erase Spot Tool
This function removes any extra spots that were counted either automatically or
manually. For example, if a portion of an AOI has high background or other noise, a
number of objects may erroneously be included in the count. Draw a box around the
object or set of objects. Objects will be displayed with a white background to indicate
that they are selected. Clicking Erase Spot deletes all selected objects at once and
automatically reduces the total count information.
Spot Count Data
When AphaEaseFC opens, the Object Count data box is hidden. To display spot count
data, click the checkbox to deselect Hide Data. The following data window will appear:
Auto Count Data Window Showing AOI Summary Data
This window shows the summary data for the area(s) of interest (AOIs). This includes
the threshold values that were set for each count type, the number of objects found for
each count type, and the total pixels in all of the spots.
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