Chapter 5: Analysis Tools
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Reset Base
Activating the Reset Base function will move the baseline to the 0 position (no
background). Unlike Horizontal Base the baseline cannot be moved.
Base Subtract
This selection will subtract the current baseline from the intensity value line. Un-check
Base Subtract to deactivate this feature.
Interpreting 1D-Multi Data
Alpha Ease™ provides a variety of tools to help interpret 1D scan data. These tools are
found in the toolbox at the bottom of the screen, and in the Data pull down menu in each
graph window.
Data Interpretation Tools
V. Line
Typically, the peaks on a graph correspond to the bands in a lane. The V. Line tool can
help determine if the peaks on two different graphs represent bands at the same molecular
weight position (i.e. the same distance from the top of the gel).
To compare the positions of peaks on different graphs, click on the V. Line checkbox. A
vertical line which can be moved across the graphs will appear. Use this line to
determine if two peaks are located in the same position
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