Mitsubishi P91DW User Manual download pdf (Page 113)

Alpha Innotech User’s Manual, version 4

5-12

Once the file has been selected, click on the OK button to load the file. (Alternatively,

double-click on the file name.) The dialog box disappears and a new dialog box appears.

Auto Load Dialog Box

This box gives the file name selected, as well as the number of markers contained within

the file. It also give the user the option of auto-loading the file.

If Yes is selected, a vertical line will appear attached to the cursor. Place the vertical line

in the marker lane on the gel, and click. AlphaEaseFC will automatically search for the

markers in the lane. In the above example, the brightest 7 bands in the lane would be

selected, and the values for Boe II’s 100 bp DNA marker ladder would be assigned to the

bands.

If No is selected, AlphaEaseFC will display a cursor with a red line attached. Text in the

upper right-hand corner of the image window will tell the user how many markers have

been entered, and the value of the next marker to be entered.

Manual Load Dialog

To begin entering markers, move the cursor to the top band in the lane and align the

horizontal line with the band. Click the left mouse button. Notice that the position for that

band is added to the markers data table.

Move the cursor to the next band, click the left mouse button again to assign the next

value in the series. Repeat this process until the values of all the marker bands are

assigned.

To quit this function before all markers have been placed, click the right mouse button.

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Mitsubishi P91DW User Manual Page 113