Mitsubishi P91DW User Manual download pdf (Page 120)

Chapter 5: Analysis Tools

5-19

The Invert check box should be selected if the image being analyzed is a dark sample on

a white background. These are usually colorimetric gels such as Coomassie Blue.

Reversing the image on the Contrast Adjustment window would NOT require the user to

select invert.

Specifying the Scan Width

The Scan Width (the green line within each lane) is the area from which the pixel

density is measured. The control labeled WIDTH sets the Scan Width. The number in

the center of this control indicates the current Scan Width setting. It can be adjusted by

clicking on the right (increase) or left (decrease) arrows. As the setting is changed, the

template displayed on the image is updated to reflect the changes.

The density values for 1D-Multi graphs are determined by the average pixel density

between the green lines on the template. A scan width that is too narrow may not include

enough information and can result in a noisy graph. By contrast, a wide scan width can

incorporate background pixels that will reduce the pixel average and dilute the actual

data. Therefore, to generate the most meaningful quantitative data, set the width to

include as much of the bands as possible without including regions of background. The

scan width should not exceed the band edges and cannot exceed the red lane limit.

Scan Width of 3 = Too Narrow (data noisy and graphs jagged)

Scan Width of Entire Lane = Too Wide (peak heights reduced due to inclusion of

background pixels)

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Mitsubishi P91DW User Manual Page 120