Mitsubishi P91DW User Manual download pdf (Page 136)

Chapter 5: Analysis Tools

5-35

The Mass for all bands is now calculated based on the total mass entered for the MW

standard lane and reported in one report.

Note Legend: Describes which lanes/bands make up the

MW and Mass standard curve values. Also notes which

regression method has been used to calculate the MW and

Mass curves.

mws: Molecular Weight Marker

ms: Mass Standard Marker

1 2 ... 131 132 133 134 135 136 137 138 139 140 141 ... 205 206

AlphaImager™ IS-2200 1
For Windows 2000/XP 1
AlphaImager 2
2200 – 2
AlphaEaseFC User’s Manual 2
1.2.1 System Components 8
1.2.2 System Placement 8
1.2.3 Cable Connections 8
1.5 System Information 15
1.6 Acquiring an Image 16
1.6.1 Focusing on an Image 17
Selecting a Filter 21
Displays 23
Imaging Functions 32
2.1 Contrast Adjustment 33
Black Level Adjustment 34
White Level Adjustment 35
Gamma Setting Adjustment 35
The Auto Contrast Selection 36
The Reverse Button 37
The Grid Button 37
2.2 Tool Bar 40
Sample Printouts 42
2.3 Tool Box 43
2.4 Status Bar 43
Chapter 3: Drop Down Menus 46
File Open 47
File Overlay 48
File Close 48
File Save and Save As 49
File Types: 50
Chapter 3: Drop Down Menus 52
3.2 The Edit Menu 53
Reset and Clear 54
3.3 The Image Menu 55
Conversion 56
Flat Field Calibrate 57
3.4 The Setup Menu 59
Load Defaults 60
Preferences 63
3.5 The Overlay Menu 66
3.6 The Utilities Menu 69
Notepad 70
3.7 The View Menu 71
Zoom Functions 72
Show Annotations 72
3.8 The Help Menu 73
Chapter 4: The Image 76
4.2 The Rotate / Flip Tool 77
4.3 Histogram 78
4.4 Automatic Enhancement 79
4.5 Annotations 81
Chapter 4: Enhancement Tools 82
Text Background and Font 83
Text Angles 84
The Drawing Tools 84
The Editing Tools 86
4.6 False Color 88
Palettes 2 through 11 89
4.7 Image Filters 90
Sharpening Filters 92
3-D (Contour) Filters 92
Noise Filters 93
Smoothing Filters 93
Edge Filters 93
Horizontal Edge Filter 93
Vertical Edge Filter 94
Custom Filter 94
The UNDO Button 94
Examples of Filter Results 94
4.8 Movie Mode 95
Frame Stacking 98
BLANK PAGE 100
Tools in the Toolbox 102
Setting up an ARRAY Template 103
Aligning the Template 104
Measuring Density 105
The INVERT Box 106
Outputting Data 106
Output Dialog Box 107
Introduction 108
Chapter 5: Analysis Tools 110
Manually Selecting Bands 111
Automatic Band Finding 111
Special Functions: 115
Snap to Peak 115
The INVERT Button 115
Calculating R 116
Values 116
The Graph Tool 117
Auto Grid 118
Setting up the Lane Template 119
Specifying the Scan Width 120
Scanning the Image 121
Graph Display 122
The Active Graph 122
The Data Window 123
Peak Detection Algorithms 125
Set parameters dialog box 129
Reset Base 130
Base Subtract 130
Interpreting 1D-Multi Data 130
V. Line 130
3-D View 131
Smooth Data 131
The Invert Button 132
Overlaying Graphs 133
1D-Multi 134
Band Scoring 139
Outputting Quantitative Data 141
Exporting Graphs 141
Exiting 1D-Multi 142
Auto Lane 142
Black Bands or White Bands 142
Sensitivity Adjustment Bar 143
Area of Interest Drawing 143
Output Data 146
Baseline 147
Options 147
Editing Peak Boundaries 147
Auto Lane Editing Features 148
Manual Band Matching 156
Band Matching Results 157
5.4 Spot Density Tools 161
Magic Wand and AutoSpot 163
Magic Wand: 163
Magic Wand Sensitivity 163
Area of Interest 165
Bright Spot vs Dark Spot 166
Find Spots 166
Background Threshold 166
Moving Objects 168
Deleting Objects 168
Saving and Loading Objects 168
Object Libraries 169
Spot Density Measurements: 169
Data Definitions 170
Inverting the Data 170
No Background 171
Automatic Background 171
Manual Background 172
The Standard Curve Toolbox 173
Displaying the Curve 175
Outputting Quantitation Data 176
Manual Count Tools 177
Auto Count Tools 177
Manual Count 179
Automatic Count 180
Editing Tools 182
Displaying Spots 183
Using the ADD SPOT Tool 183
Using the Erase Spot Tool 184
Spot Count Data 184
Outputting Auto Count Data 185
5.6 The Scoring Function 187
Scoring the Sample 188
5.7 The Ruler Function 190
Appendix A: Opening 192
AlphaEaseFC Files 192
Weight Library Files 194
To Change the Security Setup 196
Security Features Dialog Box 197
Instructions 202
Cabinet Setup 203
Camera Installation 206

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