Mitsubishi P91DW User Manual download pdf (Page 23)

Chapter 1: Introduction and Setup

1-18

1.6.6 Optimizing the Gray Scale for Saturation and Contrast

Displays

If an image will be analyzed, it is important that it not be over-saturated (too light) or

under-exposed (too dark). Using Show Saturation, the user can see the areas of the

image that are assigned to each end of the gray scale spectrum, and can adjust the

imaging controls accordingly.

The Show Saturation checkbox (found below the camera control functions) allows the

user to access the Saturation Palette during image acquisition. The Saturation Palette

is a modified gray scale palette in which black (gray level 0) is replaced with green, and

white (gray level 255, 4,095, or 65,535) is replaced with red. With this palette, over- and

under-exposed areas of the image are shown as green or red, while areas within the linear

range of the CCD chip are shown in gray scale.

During image acquisition, note regions of the image that appear red or green. Adjust the

exposure time and/or the camera aperture to minimize the amount of red and green in the

image area. If an image will be quantified, it is especially important that the actual

sample area be neither red nor green. Once you are satisfied with the displayed image,

you may click off the Show Saturation.

The other three selections Chemi Display, Auto Contrast, and Reverse are

visualization tools designed to enhance contrast and provide flexibility in regards to how

the user wishes to view the image. Auto Contrast will display the image with automatic

black, white and gamma adjustments according to the image histogram information

(black/white levels). Reverse will show the sample as a negative by switching the black

and white values. Chemi Display utilizes both the auto contrast and reverse functions as

well as adjusting for gamma. It is intended for use with chemiluminescent samples.

These options are also available in the Movie portion of the acquisition software. It is

important to note that these Contrast Display options are only visualization tools and are

NOT changing the image data that is being acquired by the CCD camera.

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Mitsubishi P91DW User Manual Page 23