Chapter 5: Analysis Tools
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the file to be saved in the directory of interest. Select apply to calculate the unknown
values of the markers in the rest of the lanes.
Saving a Mass Standard file in Autogrid…
And in Autolane
Mass Standard files can be loaded by selecting Add Mass Std in either autolane or
autogrid. Browse through the directories to find the marker file of interest, then select the
file name and click on open. The user will have the option to add the markers manually
or to auto-load the markers. Markers can be auto-loaded by selecting yes in the dialog
box and then pointing to the appropriate lane on the gel in autolane, or the appropriate
lane profile in autogrid. To add the markers one at a time, select no in the dialog box and
then point to the individual bands and click once on the left mouse button on the band of
interest. Continue to do this until all of the markers have been loaded. Select apply to
calculate the unknown mass values in the rest of the image.
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