Mitsubishi P91DW User Manual download pdf (Page 111)

Alpha Innotech User’s Manual, version 4

5-10

To delete all the markers and start over, select the Clear Markers function in the Marker

menu.

Determining Molecular Weights of Unknown Bands

After marker values are entered, the molecular weight of any unknown band can be

determined.

Manually Selecting Bands

To indicate unknown bands manually, select the Add Band function from the Query

menu. Just as in Add Marker above, a line will appear attached to the cursor. Point the

cursor at the band of interest and click the mouse button. The molecular weight of the

band is automatically calculated and displayed in the Queries section of the data box.

To select a second band, click the right mouse button and the cursor line will reappear.

Repeat this procedure for all bands for which molecular weights is to be determined.

Note that the molecular weight markers appear in blue while the queries appear in green.

The molecular weight of a band is calculated based on the graph of the known marker

bands. (Note: If a query band lies outside of the markers, it will be extrapolated in “Least

Squares Fit” mode or given a value of “N/A” in “Point-to-Point” mode.) See The

Graph Tool below for more information.

Automatic Band Finding

AlphaEaseFC includes an algorithm for automatically finding bands in query lanes. To

use this function, select Auto Query from the Query menu. A yellow vertical line will

appear attached to the cursor. Position the line over the lane of interest and click the left

mouse button. The bands in the lanes will be selected automatically, and their data will

appear in the Data box.

Repositioning and Deleting Bands

Bands can be repositioned simply by positioning the cursor, clicking, and dragging the

band to the desired location.

1 2 ... 106 107 108 109 110 111 112 113 114 115 116 ... 205 206

No comments

Mitsubishi P91DW User Manual Page 111