Alpha Innotech User’s Manual, version 4
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This method requires that you have performed band detection first. The background is
taken as the line between the edges of the bands in the lane. You enter a value (see below
in setting parameters) of maximum slope in the accompanying entry box to avoid
situations where the edge between overlapping bands, which is not at the background
intensity, causes the background to climb too high.
Rolling Disc
This method requires you to enter a parameter for the size of the disc (see below in
setting parameters) in the entry box. This option calculates the background as if a disc,
with the radius you have entered, were rolling underneath the lane profile. The larger the
radius of the disc, the less the background rises with the profile.
Set Parameters:
Set parameters dialog box
Above dialog box is used to change values for Rolling Disc and Valley to Valley
background methods.
Base Lock
The Base Lock function will designate the active graph baseline for all of the lanes in
the data table. To de-select this option click on the Base Lock a second time. The check
mark will disappear.
Horizontal Base
Activating the Horizontal Base function will move the baseline to the 0 position (no
background). The baseline can be moved vertically by clicking the baseline and dragging
it upwards.
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